Archives → 2022 → Vol. 62, № 3 → pp. 264-272
Article
Increasing the Radiosensitivity of MCF-7 Cancer Stem Cells Cultivated as Mammospheres to γ- and γ-neutron Irradiation with Metformin
Shuvatova V.G., Kuvyrchenkova A.P., Semochkina Yu.P., Moskaleva E.Yu.
National Research Center Kurchatov Institute, Moscow, Russia
Abstract
Cancer stem cells (CSCs) are highly resistant to chemo- and radiotherapy. The aim of the work was to study the effect of metformin on the radiosensitivity of MCF-7 cells cultivated as mammospheres enriched with CSCs. One hour after the addition of metformin at a non-toxic concentration of 0.25 mmol/l, cells of mammospheres were exposed to γ-radiation at a dose of 2 Gy or reactor &gamma, n-radiation at a dose of 1 Gy. After 24 hours of cultivation, the drug was removed. Six days after irradiation, the total number of cells was calculated by haemocytometer, then the percentage of CSCs with the CD44+/CD24-/low phenotype was determined, and the number of CSCs in the samples was calculated. The exposure to &gamma, n-radiation led to a significant decrease in both the total number of cells and CSCs compared with γ-radiation. The combined exposure to metformin and γ- or &gamma, n-radiation led to significant decrease in both the total number of cells and CSCs compared with the exposure to γ-or &gamma, n-radiation only (p < 0.05). Thus, &gamma, n-radiation is more effective in respect to eliminating the MCF-7 CSCs than the γ-radiation. Metformin increases the sensitivity of the MCF-7 CSCs to γ-and &gamma, n-radiation.
Keywords
Cancer stem cells, breast adenocarcinoma, MCF-7 line, mammospheres, metformin, γ-radiation, γ,n-radiation, radioresistance, radiosensitization
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