Archives → 2021 → V. 61. № 5 → pp. 451-459
Article
Chitosan matrix as a photomodulator for bromelain
S. M. Pankova1,2, M. G. Holyavka1,3, M. S. Kondrat'ev4, Yu. M. Vyshkvorkina5, A. N. Lukin1, V. G. Artyukhov1
1Voronezh State University, Voronezh, Russia 2Voronezh State Medical University named after V.I. N.N. Burdenko, Voronezh, Russia 3Sevastopol State University, Sevastopol, Russia 4Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia 5Moscow Institute of Physics and Technology, Moscow, Russia
Abstract
It was shown that when exposed to UV radiation the activity of free bromelain (EC 3.4.22.4) increases by 86% at a dose of 151 J/m2. The decrease in catalytic capacity by 15% is recorded when a dose of 6040 J/m2 is used. The change in the size (radius) of the enzyme molecule was not established in the dose range 151–6040 J/m2. After the adsorption of bromelain on the matrix of high molecular weight (350 kDa) chitosan, the activity of the enzyme irradiated in the entire range of doses used by us remained within 97%, after immobilization on medium molecular weight (200 kDa) chitosan – up to 89%. In the analysis of the infrared spectra of the immobilized bromelain it was revealed that the characteristic bands of amide I, amide II, amide III, no significant changes, consequently, it can be assumed that the chitosan matrix will act as photomodulator for the enzyme adsorbed thereon.
Keywords
IR spectroscopy, bromelain, chitosan, adsorption immobilization, UV irradiation
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