Radiation biology. Radioecology
ISSN: 0869-8031

Archives → 2020 → V. 60. №4. → pp. 411–417

Article

Investigation of UV Modification Processes for Free and Immobilized on the Chitosan Matrix Ficin

C. M. Pankova^a, M. G. Holyavka^a, M. S. Kondratyev^c, Yu. M. Vyshkvorkina^b, A. N. Lukin^a, and V. G. Artyukhov^a

^aVoronezh State University, Voronezh, Russia ^bMoscow Institute of Physics and Technology, Moscow, Russia ^cInstitute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia

Abstract

It was established that under the action of UV irradiation at doses of 151–1510 J/m2, the activity of free ficin (EC 3.4.22.3) and the diameter of its molecules remained at a constant (initial) level. When using doses of 3020, 4530, 6040 J/m2, a decrease in the catalytic ability of the enzyme and an increase in the diameter of its globule were registered, which is probably due to the unfolding of the enzyme molecule. After adsorption of ficin on the matrix of medium molecular weight (200 kDa) and high molecular weight (350 kDa) chitosans, the catalytic ability of the enzyme preparation did not decrease in the whole range of UV doses used by us. It can be assumed that the chitosan matrix acts as a photoprotector for ficin immobilized on it, since the changes in the IR spectra of the cysteine protease adsorbed on it practically do not affect the bands caused by the protein component of the system: amide I, amide II, amide III.

Keywords

UV irradiation, adsorption immobilization, ficin, chitosan, IR spectroscopy