Radiation biology. Radioecology
ISSN: 0869-8031

Archives → 2020 → V. 60. № 1. → pp. 26-33

Article

Experience of Application of Syngeneic Multipotent Mesenchymal Stem Cells (MMSC) Adipose Tissue for Treatment of Severe Radiation Skin Lesions at Various Intervals after Exposure in the Experiment

Yu. B. Deshevoi¹, T. A. Nasonova¹, O. A. Dobrynina¹, R. V. Deev², V. G. Lebedeva¹, A. V. Lyrshhikova¹, T. A. Astrelina¹ and B. B. Moroz^{1 1}Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency of Russia, Moscow, Russia ²Human Stem Cells Institute, Moscow, Russia

Abstract

The work summarizes data obtained in experiments where we studied the effectiveness of syngeneic multipotent mesenchymal stem cells (MMSC) adipose tissue when treating severe radiation skin lesions. Experiments were carried out on rats inbreed line Wistar-Kyoto, which subjected the local effects of X-ray radiation in the back in a dose of 110 Gy (30 kV tube voltage, amperage 6.1 mA, filter 0.1 mm Al), dose rate of 17.3–20.0 Gy/min. Local exposure led to the development of severe long-term (up to 3.5 months) healing Ray skin ulcers. MMSC injected under the skin around the kill zone at various intervals after exposure: in the early period prior to the formation of ulcers, ulcers and the period established during periods of active regeneration in the affected tissue. Carried out as single and double cell transplantation. It is shown that only double introduction MMSC (with an interval between injections to 1 week) increase regenerative processes and accelerates
healing of radiation ulcers. Therapeutic effect is more pronounced in terms of double imposition MMSC during the period when the radiation ulcer already formed and in the affected skin in the affected skin activates regenerative processes.

Keywords

X-ray radiation, radiation skin ulcers, multipotent mesenchymal stem cells of adipose tissue, cleprecise therapy