Archives → 2018 → Vol. 58, No. 5 → pp. 511-516
Article
Initiator Caspases-8 and -12 in the Processes of Realizing Selected Stages of Human Lymphocyte Apoptosis by UV-Radiation
Nakvasina M.A., Tokmakova E.V., Efremova D.S., Artyukhov V.G.
Voronezh State University, Voronezh, Russia
Abstract
Based on the analysis of the changes in the level of the functional activity of human lymphocyte initiator caspases-8 and -12 after exposure to UV radiation (240–390 nm) at a dose of 1510 J/m2, we have shown the possibility of their participation in the realization of the receptor caspase pathway (caspase-8) and signaling pathways associated with disruption of calcium homeostasis and changes in the level of secondary messengers such as Ca2+ and cAMP (caspase-12). The flow cytometry method has shown that 4 hours after UV radiation of lymphocytes, the number of cells in the early stage of apoptosis increased in comparison with the control by 20%. This data can be explained by the activation of «fast-acting» apoptosis signaling pathways leading to translocation of phosphatidylserine to the cell surface.
Keywords
lymphocytes, UV-radiation, apoptosis, ways of realization of apoptosis, initiator caspases, caspase-8, caspase-12
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