Archives → 2022 → Vol. 62, № 5 → pp. 532-542
Article
Chitosan and Chitosan Succinate as Photoprotectors for Papain Immobilized on Their Matrix
Holyavka M.G.1, 2, Pankova S.M.1, 3, Vyshkvorkina Yu. M.4, Lukin A.N.1, Kondratyev M.S.5, Artyukhov V.G.1
1Voronezh State University, Voronezh, Russia 2Sevastopol State University, Sevastopol, Russia 3N.N. Burdenko Voronezh State Medical University, Voronezh, Russia 4Moscow Institute of Physics and Technology, Moscow, Russia 5Institute of Cell Biophysics, Russian Academie of Sciences, Pushchino, Russia
Abstract
It has been established that UV radiation in doses of 453–6040 J/m2 has an effect on the decrease in the specific activity of free papain. Adsorption immobilization on a matrix of medium-molecular weight (200 kDa), high-molecular weight (350 kDa), food (<100 kDa) chitosans and chitosan succinate promotes an increase in the stability of papain molecules with respect to UV irradiation in the dose range from 151 to 6040 J/m2 compared to free enzyme. Modifications in the IR spectra of immobilized papain practically do not affect the bands caused by the protein component of the system: amide I, amide II, amide III, so it can be postulated that the chitosan and chitosan succinate matrix act as a photoprotector for immobilized papain. The results obtained can be useful for pharmacology and medicine in the development of drugs that promote accelerated healing of the skin, as well as in the selection of conditions for UV sterilization of drugs containing papain.
Keywords
UV-irradiation, adsorption immobilization, gel incorporation, papain, chitosan, chitosan succinate
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