Archives → 2022 → Vol. 62, № 5 → pp. 495-501
Article
Effect of Deuterium Oxide on recA and colD Genes Expression Induced by UV Radiation in Esherichia coli Cells
Abilev S.K.1, 2, Smirnova S.V.1, Shapiro T.N.1
1Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, Russia 2Department of Genetics, Lomonosov Moscow State University, Moscow, Russia
Abstract
It was shown for the first time that deuteration of Esherichia coli bacteria with deuterium oxide (D2O) at concentrations from 5 to 10% in the medium leads to an enhancement of the SOS response in the biosensors E. coli MG1655 (pColD::lux) and E. coli MG1655 (pRecA::lux) induced by UV at a dose of 12 J/m2. The biosensors used in this work contain hybrid plasmids carrying the luxCDABE operon of the Photorhabdus luminescens photobacterium, placed under the control of the cda (colD) and recA genes promoters. The induction of genotoxic factors in biosensors indicates the activation of the corresponding E. coli SOS system genes expression. A simultaneous study of the luminescence intensity and the cell viability of E. coli MG1655 biosensor (pCol-lux) bacteria showed that UV irradiation at a dose of 12 J/m2 increases the luminescence intensity from 2111.1 in a medium without D2O to 5030.3 RLU for 107 viable cells in a medium with D2O. The luminescence intensity of the biosensor was 2.5 times higher in a medium with deuterium than without it.
Keywords
Biosensors, Esherichia coli, deuterium oxide, UV irradiation, SOS response, luminescence
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