Archives → 2022 → Vol. 62, № 4 → pp. 399-407
Article
Control Levels of FISH-Registered Translocations: Literature Review
Lomonosova E.E., Nugis V.Yu., Nikitina V.A., Kozlova M.G.
State Research Center − Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russia
Abstract
This paper presents a review of the information published in the scientific literature and relating to the estimation of spontaneous levels of translocations detected by various methods of FISH-staining of chromosomes in the human peripheral blood lymphocyte cultures. Different authors using single, two and three-co-lor variants are generally indicated by a significant increase of these chromosome aberration frequencies with an increase of donor age and the absence of gender influence. An increase of translocation yield was recorded for persistent smokers and, apparently, alcoholics. It was recognized impossible to divide the effect of the race and the territorial position of research laboratories. At the same time, countries can differ significantly in terms of the lifestyle of the population, the background impact of ionizing radiation and used medical services, in particular, in terms of the volume of implemented radiation diagnostic researches. It limits the use of spontaneous damage levels of chromosomes determined by international studies and indicates desirability to have relevant own data for individual populations. At the same time it is necessary to state the limitation of similar information about the frequencies of translocations detected with multicolored FISH-staining in the control groups of people.
Keywords
Peripheral blood lymphocytes culture, FISH-staining, translocations, control levels
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