Archives → 2022 → Vol. 62, № 1 → pp. 28-41
Article
The Connection Between the Growth Dynamics of Transplanted Lewis Carcinoma in Mice and Changes in the Activity of Genes and Non-Coding RNAs after Low-Dose Irradiation
V. F. Mikhailov1, D. V. Saleeva1, L. V. Shulenina1, N. F. Raeva1, L. M. Rozhdestvensky1, G. D. Zasukhina1,2
1State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russia 2The Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia
Abstract
The effect of low-dose radiation was studied on the growth dynamics of transfused Lewis carcinoma cells in female C57Bl/6 mice. Four-fold total fractionated irradiation was performed at a dose of 75 mGy (at a dose rate 0.154 Gy/min and a voltage of 200 kV), starting from 10 days after transplantation with a four-day interval. On 14 and 22 days, a group of mice was euthanized to study the expression levels of genes and non-coding RNAs (microRNAs and long non-coding RNAs) in tumor cells, as well as in normal tissues (bone marrow, liver, spleen). Total RNA was obtained and complementary DNA was synthesized according to the manufacturer’s protocol. After that, a real-time PCR reaction was performed using a SYBR Green I dye (Thermo Scientific, USA) or a TaqMan probe and specific primers. Genes and non-coding RNAs were divided into groups oncogenes and oncosuppressors according to scientific researches. In the group of irradiated mice, a decrease in the tumor growth rate was observed, mainly from the 20th day, accompanied by a change in the activity of the studied genes. The ratio of oncosuppressors’ activity to oncogenes was calculated for the obtained tissues. These fractions were 0.5 for the tumor, 10.7 for the bone marrow, and 2.4 for the spleen and thymus. Thus, it can be stated that in the tumor there was a particularly pronounced activation of oncogenes compared to the activity of oncosuppressors, while the opposite effect was observed in normal tissues.
Keywords
low-dose radiation, Lewis lung carcinoma, genes, non-coding RNA, oncogenes, oncosuppressors, p53, NFkB
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