Archives → 2022 → Vol. 62, № 1 → pp. 5-17
Article
Analysis Peripheral Blood Lymphocytes Cultures of Patient in Long Terms after Emergency Irradiation with the Help of Three-Color FISH Method
E. E. Lomonosova1, V. Yu. Nugis1, G. P. Snigiryova2, M. G. Kozlova1, V. A. Nikitina1, I. A. Galstyan1
1Государственный научный центр Российской Федерации – Федеральный медицинский биофизический центр им. А.И. Бурназяна ФМБА, Москва, Россия 2Национальный медицинский исследовательский центр нейрохирургии им. акад. Н.Н. Бурденко Министерства здравоохранения Российской Федерации, Москва, Россия
Abstract
The results of cytogenetic analysis using mainly three-color FISH-staining of chromosome peripheral blood lymphocytes of one patient are presented in this paper. This patient was subjected to irradiation as a result of the accident at the Chernobyl nuclear power plant. The initial dose estimated by the dicentrics frequency was 3.2 Gy. The material was obtained in the total time interval of 9053–12554 days (24.8–34.4 years) after radiation exposure. The sets of DNA probes to {1, 4, 12} and {2, 3, 8} pairs of chromosomes were used, but not always together in all times. Total genomic frequency of complete and incomplete translocations was not statistically significantly changed for the entire period of observation. Retrospective dose estimates were produced according to the previously obtained in vitro dependences of the dose-effect. Dose values were located in the ranges from 1.98 to 2.44 Gy and from 2.14 to 2.67 Gy when analyzing of metaphases of all cells and only stable cells, respectively. The problem of taking into account of the presence of cell clones with chromosomal translocations is discussed.
Keywords
peripheral blood lymphocyte culture, acute irradiation, tricolor fish method, translocations, retrospective assessment of radiation dose
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