Archives → 2023 → Vol. 63, № 4 → pp. 394–402
Article
The Comet Assay Did Not Reveal a Decrease in DNA Damage to Lymphocytes when Exposed to X-Ray Radiation under the Action of Na-Cu-chlorophyllin
L. A. Romodin, M. A. Ignatov
State Scientific Center of the Russian Federation – Federal Medical Biophysical Center Named after A.I. Burnazyan of the FMBA of Russia, Moscow, Russia
Abstract
The search for effective but non-toxic radioprotective agents remains the main task of radiobiology. According to a number of reports, these may include preparations based on chlorophyll, in particular, chlorophyllin – a water-soluble product of its saponification. Since many researchers assign DNA damage a key role in the development of negative consequences of ionizing radiation, we conducted an experiment on X-ray irradiation of a suspension of lymphocytes in solutions of sodium-copper chlorophyllin in the concentration range of 5–100 micromoles. During it, using an alkaline modification of the gel electrophoresis method of individual cells, we found no significant differences in the DNA content in the tail and the tail moment of the DNA comets of irradiated lymphocytes incubated in chlorophyllin, compared with only irradiated cells. We explain this result by the fact that, most likely, chlorophyllin does not pass into the cell nuclei. And therefore it cannot show its antioxidant role in them.
Keywords
Ionizing radiation, lymphocytes, chlorophyllin, radioprotectors, comet assay, DNA
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