Archives → 2022 → Vol. 62, № 2 → pp. 164–170
Article
Changes in the Concentration of Extracellular DNA and Leukocytes in the Peripheral Blood in the Early Stages of Development of Radiation Cystitis in Rats
I. N. Vasilyeva1, O. V. Korytov2, S. D. Ivanov1, A. L. Semenov1, V. G. Bespalov1, L. I. Korytova2
1N.N. Petrov National Medical Research Center of Oncology, St.-Petersburg, Russia 2A.M. Granov Russian Scientific Center for Radiology and Surgical Technologies, St.-Petersburg, Russia
Abstract
The objective of this study was to identify an indicator that will change early after irradiation and can be used for early (within 6–24 hours) diagnosis of cystitis initiation as a complication arising from radiation therapy of tumor carriers in the pelvic area. On a newly developed model of radiation-induced cystitis, irradiation of the rat urinary bladder area was carried out on a linear electron accelerator Axesse (Elekta, Sweden) with a quantum energy of 6 MeV at a dose of 25 Gy at a dose rate of 4.50 Gy/min once. Early effects after a single local irradiation at a dose of 25 Gy to the pelvic region in female rats was assessed by the change in the peripheral blood of the total number of leukocytes and their fractions, the concentration of extracellular DNA (exDNA), as well as cholesterol, gamma–glutamyltransferase, urea, serum alkaline phosphatase after 6 and 24 hours after irradiation. 6 h after exposure, there was a decrease in the total number of leukocytes from 16.06 ± 1.83 to 10.57 ± 1.06 (×109/L), lymphocytes from 11.44 ± 1.30 to 5.84 ± 0.47, monocytes from 0.51 ± 0.08 to 0.32 ± 0.03, and after 24 hours the number of granulocytes also decreased from 4.11 ± 0.57 to 2.14 ± 0.15. 6 and 24 h after irradiation, an increase in the concentration of exDNA in blood serum was observed from 7.70 ± 0.55 ng/μL to 11.20 ± 1.33 ng/μL, which then returned to normal by 48 hours. ExDNA is an indicator of cell death under local exposure to radiation and can be a promising early non-invasive marker of the development of radiation cystitis, useful in the future for the selection of means for preventing the onset of radiation pathology.
Keywords
local irradiation of rat urinary bladder, radiation cystitis, leukocytes and their fractions, extracellular DNA
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