Archives → 2022 → Vol. 62, № 6 → pp. 620–628
Article
Effect of γ-Irradiation of Seeds on Intravariety Variability of Quantitative Characters of Yellow Lupin
Novik N. V.1, Geras'kin S. A.2, and Yakub I. A.1
1All-Russian Lupin Scientific Research Institute – branch of the Federal Williams Research Center of Forage Production & Agroecology, Bryansk, Russia 2Russian Institute of Radiology and Agroecology, Obninsk, Russia
Abstract
Seeds of the var. Bulat (traditional morphotype) and of the BL 12-11-02-2-4-1ep (epigonal morphotype) were irradiated with the equipment GUR-12 0 (60Со) to develop mutant lines of yellow lupine. The irradiation dose/energy was as following: 200 Gy (60 Gy/hour); 300 Gy (60 Gy/hour); 400 Gy (60 Gy/hour); 200 Gy (100 Gy/hour); 300 Gy (100 Gy/hour); 400 Gy (100 Gy/hour); 300 Gy (150 Gy/hour); 400 Gy (150 Gy/hour). Statistical significant differences for the length of the main root and hypocotyl for each variant compared to the standard one were revealed in the laboratory tests. Significant differences were also revealed between variants with different energy at the irradiant dose 200 Gy; at the doses 300 and 400 Gy the differences are smoothed out. The LD50 for yellow lupine made 400 Gy at the energy 60 and 100 Gy/hour. In a field experiment the effect of pre-sowing seeds’ irradiation on seeds’ viability, on elements of plant productivity in M1 and M2 generations as well as on biochemical characteristics of their seeds is revealed. The specificity of varieties in response to seeds irradiation was revealed too. It means the increase of adaptability of the ultra-maturing breeding line 12-11-02-2-4-1ep.
Keywords
Radiation mutagenesis, yellow lupin
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