Archives → 2020 → V. 60. № 2. → pp. 153–162
Article
Research of the Effect of Proton Radiation on the Brain Proteome of Mouse
A. V. Litvinchuka,c, Е. S. Zorinab, А. Т. Коpylovb, V. О. Popovaa, О. К. Leginaa, N. L. Ronzhinaa, N. А. Verlova, J. L. Каrlina, V. V. Lysenkoa, V. F. Еzhova,d and S. N. Naryzhnya,b
aB.P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center “Kurchatov Institute”, Gatchina, Russia. bOrekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Moscow, Russia. cMUNI, Masaryk University, Department of Experimental Biology, Faculty of Science, Brno, Czech Republic. dSaint-Petersburg State University, Saint-Petersburg, Russia
Abstract
This paper presents the results of a proteomics analysis of mouse brain nerve tissue cells after a single local effect of a proton beam with an energy of 1 GeV at an absorbed dose of 3 Gy. Electrophoretic methods of protein separation as well as mass spectrometric and immune detection methods were used in the work. It was found that on day 31 after irradiation, the number of detected proteoforms and expressed genes almost doubled: 7754 proteoforms and 1591 genes after irradiation against 3652 proteoforms and 996 genes before irradiation. The data obtained indicate the plasticity of the nervous tissue response to the action of proton radiation. Especially noteworthy are changes in the profiles of proteoforms of such regulatory proteins as 14-3-3, cofilin-1, neuromodulin and gamma-enolase that are involved in reorganization of the cytoskeleton of nerve cells and regenerative processes.
Keywords
irradiation, protons, brain, proteome, cofilin-1, 14-3-3, cytoskeleton
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