Archives → 2019 → Vol. 59, No. 2 → pp. 205-213
Article
Influence of Liposomal Compositions Containing AFP and GCSF Recombinant Human Proteins on the Mice Skin Radiation Burn According to Clinical Indicators and Indices of the Skin Stem Cells
Pryakhin E.A.1, Trjapitsyna G.A.1,2, Shaposhnikova I.A.1, Tyuhay M.V.1,2, Ostroumov Yu. I.3, Shmelin P.S.4, Akleev A.V.1,2
1Urals Research Center for Radiation Medicine, Chelyabinsk, Russia 2Chelyabinsk State University, Chelyabinsk, Russia 3 Institute of Engineering Immunology, Lyubuchany, Russia 4Technomash, Moscow, Russia
Abstract
As shown on the IIIA radiation skin burn model in CD1 mice, that transdermal application of liposomes containing rhAFP; liposomes containing rhGCSF; liposomes containing rhAFP and rhGCSF on the affected area of the skin within 2 weeks after irradiation leads a significant reduction in the healing time of the burn wound according to clinical indices. The most effective was the use of liposomes containing rhAFP: the duration of the latent period increased by 70%, the duration of the wet epidermitis was reduced by 60%, the duration of the reparative phase was reduced by 60% and the total healing time of the burn was reduced by 32%, as compared to the values in the mice with untreated radiation burns. Immunnohistochemical studies have shown that application of the drugs under study leads to an increase in the survival of stem cells in the hair follicles of the skin: the most pronounced effect on histological indices was noted in the group treated with liposomes containing rhAFP and rhGCSF.
Keywords
Irradiation, mice, skin, stem cells, radiation burn, treatment, rhAFP, rhGCSF, liposomes
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