Archives → 2019 → Vol. 59, No. 3 → pp. 305-310
Article
The Effect of Deuterium on the Expression of Inducible Genes Esсherichia coli
Abilev S.K.1,2, Igonina E.V.1, Smirnova S.V.1, Rubanovich A.V.1
1Vavilov Institute General Genetics Russian Academy of Sciences, Moscow, Russia 2Faculty of Biology, Moscow State University, Moscow, Russia
Abstract
It has been shown for the first time that preliminary treatment of bacteria with deuterium (D2O) at concentrations from 2.5 to 10% in the medium leads to an increase in the expression of the recA gene induced by H2O2 at concentrations of 2.2–8.8 mmol/l. A biosensor based on the strain E. coli K12 MG1655 (pRecA–lux), luminescing as a result of activation of the promoter of the recA gene in response to DNA damage caused by H2O2, was used to compare the induction of recA in deuterated and non-deuterated (control) cultures. D2O concentrations of 5.0 and 7.5% were most effective. To explain the phenomenon, expression of the ca-talase gene in deuterated and non-deuterated cultures of E. coli K12 MG1655 (pKatA–lux) biosensor was studied. The luminescence of this biosensor results from the activation of the promoter of the katA gene in response to an increase in the concentration of H2O2 in the cell. It has been found that D2O reduces the level of expression of the katA gene, which can lead to the accumulation of H2O2, and, correspondingly, an increase in the level of DNA damage recorded by an increase in the expression of the recA gene.
Keywords
deuterium oxide, hydrogen peroxide, RecA gene expression, KatA gene expression, biosensors
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