Archives → 2019 → Vol. 59, No. 3 → pp. 286-292
Article
Expression of Gibberelline Biosynthesis and Catabolism Genesin the Embryos of γ-Irradiated Barley Seeds
Bitarishvili S.V., Bondarenko V.S., Geras'kin S.A.
Russian Institute of Radiology and Agroecology, Obninsk, Russia
Abstract
The change in the expression of gibberellins biosynthesis (HvGA3ox2) and catabolism (HvGA2ox3) genes in the embryos of γ-irradiated barley seeds at doses of 4–50 Gy was studied during 30 hours of germination. It was shown that transcription activity of the gibberellin catabolism gene HvGA2ox3 was predominantly increased at each stage of germination, except 12 h after the beginning of the experiment. The transcriptional activity of the gibberellin biosynthesis gene HvGA3ox2 was reduced during the early stages of the observation period (after 6 and 12 hours) and increased after irradiation during the later stages (after 24 and 30 hours). The peak of the transcriptional activity of the studied genes was noted in the embryos of both control and irradiated seeds 18 hours after the onset of germination. At a dose of 20 Gy, the gibberellin biosynthesis gene transcript concentration (HvGa3ox2) was maximal and exceeded the gibberellin catabolism gene transcript concentration (HvGa2ox3) by more than an order of magnitude.
Keywords
barley, γ-radiation, gibberellins, real-time PCR, gene expression
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