Archives → 2019 → Vol. 59, No. 3 → pp. 279-285
Article
Effects of Indralin on Immune-Hematological Parameters and DNA Damagein Irradiated ICR (CD-1) Outbred Mice
Bushmanov A. Yu.1, Vorobyeva N. Yu.1,2, Blokhina T.M.1,2, Andrianova I.E.1, Stavrakova N.M.1, Bychkova T.M.1,3, Nikitenko O.V.1,3, Yashkina E.I.1, Gordeev A.V.1, Karaulova T.A.1, Vorontsova M.D.1, Ignatov M.A.1, Osipov A.N.1,2, Ivanov A.A.1,3,4
1 State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russia 2 N.N. Semenov Institute of Chemical Physics Russian Academy of Sciences, Moscow, Russia 3 Federal State Budgetary Institution of Science “Russian Federation State Scientific Center – Institute of Biomedical Problems” Russian Academy of Sciences, Moscow, Russia 4 Joint Institute for Nuclear Research, Dubna, Russia
Abstract
Intragastric administration of radioprotector indralin (drug B-190) to outbred ICR (CD-1) mice 15 minutes before X-ray irradiation at doses of 6 Gy (LD-10/30), 6.5 and 7 Gy (LD-100/30) increases the 30-day survival of the animals, positively affects the state of the central organs of immunity and hematopoiesis, as well as the number of peripheral blood leukocytes. In addition, a positive effect on the behavioral reactions of the irradiated animals and striated muscle strength was noted. In addition to these secondary features of the positive effect of indralin, a significant reduction of radiation-induced DNA damage in splenocytes was observed using flow cytometry analysis of phosphorylated histone Н2АХ (γН2АХ) (1 h and 8 days after X-ray irradiation) and DNA comet assay (8 days after X-ray irradiation). The above methods are recommended for experimental usage to investigate the anti-radiation drug influence on the DNA damage induction and repair in the irradiated organism, as well as for initial searching of potential radioprotectors and assessment of their effectiveness level in the clinical practice and experiments.
Keywords
indralin, radiation, survival, immunity, hematopoiesis, DNA-comet assay, phosphorylated histone H2AX (γ H2AX), DNA double strand breaks
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