Archives2019Vol. 59, No. 3pp. 255-260

Article

Changes in the Composition and Fluorescent Properties of Bisretinoids in the Retina and Retinal Pigment Epithelium of the Mouse Eye by the Action of Ionizing Radiation

Yakovleva M.A.1, Lyakhova K.N.2, Utina D.M.2, Vinogradova U.V.2, Kolesnikova I.A.2, Feldman T.B.1,3, Ostrovsky M.A.1,2,3

1 Emanuel Institute of Biochemical Physics Russian Academy of Sciences, Moscow, Russia 2 Laboratory of Radiation Biology, Joint Institute for Nuclear Research, Dubna, Russia 3 Department of Molecular Physiology, Biological Faculty Lomonosov Moscow State University, Moscow, Russia

Abstract

Fluorescence and chromatographic analysis of bisretinoids from the retina and retinal pigment epithelium of mouse eyes was carried out before and after exposure to accelerated protons in the Bragg peak. It is shown that the action of ionizing radiation at the doses of 1–4 Gy leads to a shift in the short-wave region of the ma-ximum of the fluorescence spectrum in the chloroform extract obtained from both the retinal pigment epithelium and the retina. Chromatographic analysis of these extracts has shown a change in the relative content of individual bisretinoids. The obtained spectral and chromatographic data indicate that exposure of the mice to accelerated protons in the Bragg peak at the doses of 1–4 Gy leads to the radiation oxidation of bisretinoids in the eye tissues.

Keywords

ionizing radiation, eye, retina, retinal pigment epithelium, bisretinoids

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