Archives → 2018 → Vol. 58, No. 3 → pp. 245-250
Article
UV-Induced Genetic Instability of Yeast Cells
Evstratova E.S.1, Khryachkova A.V.1, Zhurakovskaya G.P.1, Petin V.G.1, Podobed M. Yu.1
1 A. Tsyb Medical Radiological Research Center - branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Obninsk, Russia
Abstract
The data for survival and delayed appearance of the colonies exemplifying genetic instability are presented for haploid and homozygous diploid strains of wild-type Saccharomyces cerevisiae yeast cells surviving radiation exposure to UV-light (254 nm). The degree of genetic instability was shown to be mainly determined by cell ploidy: diploid cells in contrast to haploid ones demonstrate a large extent of the delayed appearance of clones surviving after irradiation (100% vs. 30%). Since both haploid and diploid cells displayed a sigmoid shape of dose-effect curves after irradiation with UV-light, it is concluded that a delayed appearance of colonies is mainly determined by cell ploidy rather than a sigmoid shape of the survival curve as it is traditionally assumed for Saccharomyces cerevisiae yeast cells. To identify general patterns, the data obtained are quantitatively compared with our previously published results obtained after irradiation with γ-rays and α-particles. The outcome indicates that isoeffective exposure to radiation of various nature induces an equal number of effective (lethal) damage and a proportional number of accompanying sub-lesions responsible for the delayed formation of colonies by the cells surviving exposure. Сell division disturbance, expressed by the delayed colony formation, can be considered as an example of genetic instability.
Current Issue
FAQs
No. There is no publication fee.
Yes. Most of the journals accept submissions via e-mail .