Archives → 2018 → Vol. 58, No. 3 → pp. 229-237
Article
Analysis of the Complex DNA Damage Structure after 11B Ion Irradiation and 60Co γ-Rays
Zadneprianetc M.G.1 2, Boreyko A.V.1 2, Bulanova T.S.1 2, Jezkova L.1 3, Krasavin E.A.1 2, Kulikova E.A.1 2, Smirnova E.V.1, Falk M.4, Falkova I.4
1 Joint Institute for Nuclear Research, Dubna, Russia
2 Dubna State University, Dubna, Russia
3 Institute of Chemistry and Technology, Dubna, Russia; Prague, Czech Republic
4 Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic
Abstract
The formation and structure of clustered γH2AX/53ВР1 foci induced by accelerated 11B ions (E = 7.5 MeV/n) and 60Co γ-rays in human skin fibroblasts are investigated. Slower recovery kinetics of the radiation-induced foci (RIF) in accelerated ion tracks was observed as compared to that after exposure to γ-rays due to the unique pattern of energy deposition along the particle trajectory through the cell nucleus and the induction of complex repair-resistant DNA double strand breaks (DSB). Linear energy transfer (LET) dependent changes in the structure of γH2AX/53ВР1 foci, size and shape were observed. It is concluded that formation of highly clustered foci (containing up to 6 individual γH2AX/53ВР1 foci) is a specific feature of the action of high atomic number and energy particles (HZE) on the human cell genetic structures.
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