Radiation biology. Radioecology
ISSN: 0869-8031

Archives → 2018 → Vol. 58, No. 5 → pp. 484-489

Article

The Problem of Analysis of Post-Radiation Changes in the Number of γH2AX Fociin Asynchronous Cell Population

Grekhova A.K.1,2,3, Pustovalova M.V.2,3, Eremin P.S.4, Yashkina E.I.2, Osipov A.N.2,3

1N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia. 2State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russia. 3 Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia. 4 Russian Scientific Center of Medical Rehabilitation and Health Resort of the Ministry of Public Health, Moscow, Russia.

Abstract

Quantitative analysis of phosphorylated histone H2AX (γH2AX) foci is currently used to study the patterns of radiation-induced DNA double-strand break induction and repair. In this case, the studies are often conducted on an asynchronous cell population and do not consider the fact that ionizing radiation affects the proliferative activity of the cells and their distribution in the phases of the cell cycle. In this study, a differentiated analysis of the changes in the number of γH2AX foci in non-synchronous, resting (Ki67-), proliferating (Ki67+) and S/G2-phase (CENPF+) populations of human skin fibroblasts within 24 hours after exposure to 80, 250 and 1000 mGy of X-ray radiation was conducted. It was shown that the absolute number of γH2AX foci both in the control and irradiated cells depends significantly on the proliferative activity and reaches its maximum in the cells in the S/G2 phase. Exposure to low (80 mGy) and intermediate (250 and 1000 mGy) doses of X-ray radiation differently affects the proliferative activity of the cells (stimulating and inhibiting, respectively), which may lead to the incorrect analysis of the results obtained from calculation of the number of γH2AX foci in an asynchronous cell population.

Keywords

DNA double-strand breaks, γH2AX foci, proliferation, fibroblasts, X-ray radiation

Current Issue

Vol. 63, № 4

FAQs

What is time from submission of the article to its publication?

The average period from submission to first decision in last year was 30 days, and that from first decision to acceptance was 40 days. The rejection rate for submitted manuscripts in last year was 15%.

Are the authors provided with pdf files of their articles?

Yes, authors are provided with pdf files. The files are sent to the authors' addresses specified in the file "Information about authors".

all questions


Send manuscript